TNF Receptor 1 Antibody / TNFRSF1A Polyclonal Antibody (RQ4614)

TNF Receptor 1 Antibody Immunohistochemistry. IHC staining of FFPE mouse colon tissue with TNF Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse kidney tissue with TNF Receptor 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

TNF Receptor 1 Antibody WB. Western blot testing of human 1) HeLa and 2) A549 cell lysate with TNF Receptor antibody. Predicted molecular weight ~51 kDa.

Western blot testing of 1) rat brain, 2) rat lung, 3) mouse brain and 4) mouse lung tissue lysate with TNF Receptor 1 antibody. Predicted molecular weight ~51 kDa.

Western blot testing of 1) human HeLa, 2) human K562, 3) human Caco-2, 4) rat liver, 5) mouse small intestine, 6) mouse liver and 7) mouse HEPA1-6 lysate with TNF Receptor 1 antibody at 0.5ug/ml. Predicted molecular weight ~51 kDa.

TNF Receptor 1 Antibody IHC in Human Brain Tissue. Immunohistochemical staining of formalin-fixed, paraffin-embedded human brain tissue using the TNFRSF1A Polyclonal Antibody at 2 ug/ml. Brown DAB staining is observed in scattered neuronal cell bodies, with predominantly cytoplasmic immunoreactivity and variable staining intensity. The surrounding neuropil exhibits comparatively weak, diffuse staining, while nuclei are largely negative. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. The primary antibody was incubated overnight at 4°C, and detection was performed using an HRP-conjugated secondary antibody with DAB chromogen.

TNF Receptor 1 Antibody WB in HeLa Wild-Type and TNFRSF1A Knockout Cells. Western blot analysis of human HeLa wild-type (lane 1) and TNFRSF1A knockout (lane 2) whole-cell lysates using the TNFRSF1A Polyclonal Antibody. Multiple immunoreactive bands are observed between approximately 55-70 kDa in wild-type cells, with reduced signal in the knockout sample, particularly near 55-60 kDa. Residual bands remain visible in the knockout lane. The predicted molecular weight of TNFR1 is approximately 50 kDa, although glycosylation may contribute to higher apparent molecular weights. Samples were loaded at 30 ug per lane and separated by 10% SDS-PAGE under reducing conditions. The primary antibody was used at 0.5 ug/ml, with GAPDH serving as the loading control.