TNF-alpha Antibody / TNFA [clone TNFA/1172] (V2898)

Description

TNF-alpha antibody clone TNFA/1172 is a monoclonal antibody that detects tumor necrosis factor alpha, a proinflammatory cytokine produced by macrophages, T lymphocytes, and natural killer cells. TNF-alpha plays central roles in immune regulation, inflammation, apoptosis, and host defense. It is a key mediator of septic shock and chronic inflammatory conditions, while also influencing tumor biology. NSJ Bioreagents provides this antibody for immunology, oncology, and inflammation research.

The antibody produces strong cytoplasmic and extracellular staining in immune cells activated by infection or stress. In immunology, TNF-alpha detection is essential for studying cytokine networks and signaling pathways that orchestrate immune responses. The antibody enables precise monitoring of cytokine release in models of acute and chronic inflammation.

In pathology, TNF-alpha has long been studied as a driver of autoimmune and inflammatory diseases, including rheumatoid arthritis, Crohn disease, and psoriasis. This antibody supports investigations into cytokine dysregulation in these disorders and has been applied to preclinical research on cytokine-targeted therapies.

In oncology, TNF-alpha antibody clone TNFA/1172 has been used to study how inflammatory signaling contributes to tumor initiation, progression, and metastasis. TNF-alpha can promote tumor necrosis under certain conditions but also fosters tumor growth by sustaining inflammation and angiogenesis. This dual role makes its detection important for tumor immunology and therapeutic research.

In infectious disease research, the antibody supports studies of bacterial and viral pathogens that trigger robust cytokine responses. TNF-alpha detection provides insights into cytokine storm syndromes and septic shock, where excessive signaling leads to systemic damage.

Validated across multiple assay platforms, the antibody consistently provides strong and specific detection. Alternate names include TNF antibody, cachectin antibody, and tumor necrosis factor cytokine antibody.

Application Notes

Optimal dilution of the TNF-alpha antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant full-length human protein was used as the immunogen for the TNF-alpha antibody.

Storage

Store the TNF-alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).