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Home >> Antibodies >> TNF alpha Antibody

TNF alpha Antibody [clone TMDa1-1] (V7163)

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  Catalog No Formulation Size Price (USD)  
Image V7163-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7163-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7163SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Immunofluorescent staining of PFA-fixed human HepG2 cells with TNF-alpha antibody (green, clone TMDa1-1) and Reddot nuclear stain (red).
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Immunofluorescent staining of PFA-fixed human HepG2 cells with TNF-alpha antibody (green, clone TMDa1-1) and Reddot nuclear stain (red).
IHC testing of FFPE human histiocytoma and TNF alpha antibody (clone TMDa1-1). Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE rat stomach and TNF alpha antibody (clone TMDa1-1). Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of PFA-fixed human HepG2 cells with TNF-alpha antibody (clone TMDa1-1); Red=isotype control, Blue= TNF-alpha antibody.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgM, kappa
Clone Name TMDa1-1
Purity PEG precipitation
UniProt P01375
Localization Cytoplasmic and extracellular (secreted)
Applications Immunofluorescence : 1-2ug/ml
Flow cytometry : 1-2ug/10^6 cells
Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT
Limitations This TNF alpha antibody is available for research use only.
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Description

Tumor Necrosis Factor Alpha (TNF alpha) is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. [UniProt]

Application Notes

Titering of the TNF alpha antibody may be required for optimal performance.

Immunogen

Full length human recombinant protein was used as the immunogen for the TNF alpha antibody.

Storage

Store the TNF alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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