Tnf alpha Antibody / Tnfa (RQ5771)

Description

Tumor necrosis factor alpha, commonly referred to as Tnf alpha, is a key pro-inflammatory cytokine involved in immune system regulation, cell apoptosis, and the inflammatory response. It is predominantly produced by activated macrophages, though many other immune and non-immune cell types can also express Tnf alpha under stress or pathological conditions. The signaling cascade initiated by Tnf alpha plays a central role in host defense, but its dysregulation is associated with chronic inflammation, autoimmune diseases, and cancer.

The Tnf alpha gene gives rise to multiple isoforms through alternative splicing. These isoforms may differ in their cellular localization, stability, and receptor binding capabilities. The best-characterized forms are the transmembrane precursor and the cleaved soluble cytokine, both of which are biologically active and capable of triggering downstream signaling through Tnf receptors.

A high-quality Tnf alpha antibody is essential for studying cytokine biology and immune-mediated diseases. Researchers frequently use a Tnf alpha antibody for detecting the protein in applications such as western blot, immunohistochemistry, flow cytometry, and ELISA. NSJ Bioreagents provides a validated Tnf alpha antibody designed to ensure sensitivity and specificity across these platforms, enabling researchers to explore both baseline expression and dynamic regulation of Tnf alpha under various experimental conditions.

Application Notes

Optimal dilution of the Tnf alpha antibody should be determined by the researcher.

Immunogen

Recombinant mouse protein (amino acids D89-E185) was used as the immunogen for the Tnf alpha antibody.

Storage

After reconstitution, the Tnf alpha antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.