TNF Alpha Antibody / Pro-Inflammatory Cytokine Antibody (RQ4613)

TNF Alpha Antibody Human Spleen IHC. Immunohistochemical staining of FFPE human spleen tissue with TNF alpha antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. TNF alpha immunoreactivity is observed predominantly within scattered immune cells of the splenic white pulp and periarteriolar lymphoid regions, consistent with the expected localization of this pro-inflammatory cytokine involved in immune regulation, inflammatory signaling, and activation of innate and adaptive immune responses. Cell nuclei are counterstained with hematoxylin (blue).

TNF Alpha Antibody Human Tonsil IHC. Immunohistochemical staining of FFPE human tonsil tissue with TNF alpha antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. TNF alpha immunoreactivity is observed predominantly within scattered immune cells of the lymphoid tissue and interfollicular regions, consistent with the expected localization of this pro-inflammatory cytokine involved in immune regulation, inflammatory signaling, and activation of innate and adaptive immune responses. Cell nuclei are counterstained with hematoxylin (blue).

TNF Alpha Antibody Recombinant Protein WB. Western blot testing of recombinant human TNF alpha protein with TNF alpha antibody at 0.5 ug/ml. Lane 1: 10 ng. Lane 2: 5 ng. Lane 3: 1 ng. Lane 4: 0.5 ng. A specific immunoreactive band is detected at approximately 17 kDa across the recombinant protein dilution series, demonstrating sensitive detection of TNF alpha at decreasing protein concentrations. The results confirm specific recognition of this pro-inflammatory cytokine, a central regulator of immune responses, inflammatory signaling, and cytokine-mediated host defense.