PYCARD Antibody / Inflammasome Adaptor Protein Antibody (RQ8210)

PYCARD Antibody / Inflammasome Adaptor Protein Antibody Human Intestinal Cancer IF. Immunofluorescent staining of FFPE human intestinal cancer tissue using PYCARD Antibody / Inflammasome Adaptor Protein Antibody (red) with DAPI nuclear counterstain (blue). PYCARD expression is observed predominantly within the cytoplasm of tumor cells, consistent with its role as an adaptor protein that mediates inflammasome assembly, innate immune signaling, and inflammatory responses. Heat-induced epitope retrieval was performed by steaming sections in pH 8 EDTA buffer for 20 minutes prior to immunofluorescent staining.

PYCARD Antibody / Inflammasome Adaptor Protein Antibody A549 Cell IF. Immunofluorescent staining of FFPE human A549 cells using PYCARD Antibody / Inflammasome Adaptor Protein Antibody (red) with DAPI nuclear counterstain (blue). PYCARD expression is observed predominantly within the cytoplasm, consistent with its role as an inflammasome adaptor protein that mediates innate immune signaling, ASC speck formation, and inflammatory caspase activation. Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to immunofluorescent staining.

PYCARD Antibody / Inflammasome Adaptor Protein Antibody Human Glioblastoma IHC. Immunohistochemical staining of FFPE human glioblastoma tissue using PYCARD Antibody / Inflammasome Adaptor Protein Antibody. PYCARD expression is detected predominantly within the cytoplasm of tumor cells, consistent with its role as a central adaptor protein in inflammasome assembly and innate immune signaling. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes and allowing them to cool prior to immunostaining.

PYCARD Antibody / Inflammasome Adaptor Protein Antibody Human Urothelial Carcinoma IHC. Immunohistochemical staining of FFPE human urothelial carcinoma with squamous differentiation using PYCARD Antibody / Inflammasome Adaptor Protein Antibody. PYCARD expression is observed predominantly within the cytoplasm of neoplastic cells, consistent with its established role as an inflammasome adaptor protein involved in innate immune signaling, inflammatory responses, and pyroptotic cell death. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes and allowing them to cool prior to immunostaining.

PYCARD Antibody / Inflammasome Adaptor Protein Antibody WB. Western blot analysis of human THP-1, HL-60, and A549 cell lysates using PYCARD Antibody / Inflammasome Adaptor Protein Antibody. A distinct immunoreactive band is detected at approximately 22 kDa, consistent with the predicted molecular weight of PYCARD (ASC). Strong expression is observed in THP-1 and HL-60 cells, with comparatively lower expression detected in A549 cells, supporting specific detection of endogenous PYCARD across multiple human cell lines.

PYCARD Antibody / Inflammasome Adaptor Protein Antibody THP-1 FACS. Flow cytometric analysis of human THP-1 cells using PYCARD Antibody / Inflammasome Adaptor Protein Antibody at 1 ug per million cells. A clear rightward shift of the antibody-stained population (blue) relative to the isotype control (green) demonstrates specific detection of endogenous PYCARD (ASC) expression. Cells alone are shown in red. Cells were blocked with goat serum prior to antibody incubation to minimize non-specific binding.