TLS Antibody / Translocated in liposarcoma protein / FUS (RQ6869)

IHC staining of FFPE human rectal cancer tissue with TLS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse colon tissue with TLS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat colon tissue with TLS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human A431 cells with TLS antibody (green) and Phalloidin (red). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) human HeLa, 2) human K562, 3) human 293T, 4) human Caco-2, 5) human Jurkat, 6) human HL60, 7) human HepG2, 8) human SH-SY5Y, 9) rat brain, 10) rat PC-12 and 11) mouse NIH 3T3 cell lysate with TLS antibody. Predicted molecular weight ~53 kDa but routinely observed at up to ~75 kDa.

Flow cytometry testing of human U-87 MG cells with TLS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TLS antibody.

Flow cytometry testing of mouse ANA-1 cells with TLS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TLS antibody.