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Home >> Antibodies >> TLE1 Antibody / Transducin like enhancer of split 1

TLE1 Antibody / Transducin like enhancer of split 1 [clone MSVA-856R] (V6122)

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  Catalog No Formulation Size Price (USD)  
Image V6122-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 654.50
Image
V6122-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
Microvalidated Recrabbitmono
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Transducin-like enhancer protein 1 Rabbit Recombinant Monoclonal Antibody (MSVA-856R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-856R
UniProt Q04724
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This TLE1/Transducin like enhancer of split 1 antibody is available for research use only.
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Application Notes

1. Optimal dilution of the TLE1/Transducin like enhancer of split 1 antibody should be determined by the researcher.

2. This TLE1/Transducin like enhancer of split 1 antibody is recombinantly produced by expression in human HEK293 cells.

Immunogen

Recombinant human TLE1 fragment (around amino acids 175-338) (exact sequence is proprietary) was used as the immuongen for the TLE1/Transducin like enhancer of split 1 antibody.

Storage

TLE1/Transducin like enhancer of split 1 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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