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Home >> Antibodies >> TLE1 Antibody / Transducin-like enhancer protein 1

TLE1 Antibody / Transducin-like enhancer protein 1 (F52972)

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  Catalog No Formulation Size Price (USD)  
Image F52972-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
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F52972-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Fluorescent image of HeLa cells stained with TLE1 antibody diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
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Fluorescent image of HeLa cells stained with TLE1 antibody diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot analysis of lysate from 1) MCF-7 cells and 2) human brain tissue using TLE1 antibody at 1:1000.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt Q04724
Applications Immunofluorescence : 1:25
Western blot : 1:1000
Limitations This TLE1 antibody is available for research use only.
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Description

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.

Application Notes

Titration of the TLE1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This TLE1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 187-221 amino acids from the N-terminal region of human TLE1.

Storage

Aliquot the TLE1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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