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Home >> Antibodies >> TL1A Antibody for IHC / VEGI

TL1A Antibody for IHC / VEGI [clone TLRM1-1] (V7227)

  Catalog No Formulation Size Price (USD)  
Image V7227-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V7227-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V7227SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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Immunohistochemistry of TL1A antibody in human parathyroid tumor tissue. FFPE human parathyroid lesion demonstrates diffuse cytoplasmic and membranous HRP-DAB brown staining within tumor cells, consistent with VEGI / TNFSF15 expression in endocrine-associated tissue. Clone TLRM1-1 was used as a monoclonal antibody for detection. Heat-induced epitope retrieval was performed by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to staining.
Immunohistochemistry of TL1A antibody in human colon carcinoma tissue. FFPE human colon carcinoma demonstrates strong membranous and cytoplasmic HRP-DAB brown staining within gland-forming tumor epithelial cells, consistent with VEGI / TNFSF15 localization in epithelial and vascular-associated compartments. Clone TLRM1-1 was used as a monoclonal antibody for detection. Heat-induced epitope retrieval was performed by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to staining.
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name TLRM1-1
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
UniProt O95150
Gene ID 9966
Localization Cytoplasmic, cell surface, secreted
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This TL1A antibody is available for research use only.
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Description

TL1A antibody recognizes Tumor necrosis factor ligand superfamily member 15, also known as VEGI, a cytokine encoded by the TNFSF15 gene. TL1A is a type II transmembrane protein belonging to the tumor necrosis factor ligand superfamily and is primarily expressed by endothelial cells and activated immune cells. TL1A Antibody for IHC is developed to detect endogenous VEGI in formalin-fixed, paraffin-embedded tissues for research applications focused on inflammatory signaling and angiogenic regulation.

VEGI functions as a ligand for death receptor 3, also known as TNFRSF25, activating downstream signaling pathways that regulate T cell activation, cytokine production, and apoptosis-associated mechanisms. Through this receptor interaction, TNFSF15 plays a central role in modulating adaptive immune responses, particularly in T helper cell differentiation and effector activity. In addition to immune regulation, TL1A exerts anti-angiogenic effects by inhibiting endothelial cell proliferation and influencing vascular remodeling.

The TNFSF15 gene is located on chromosome 9q32 and encodes a membrane-bound protein that can be proteolytically processed to generate a soluble form. TL1A expression has been documented in vascular endothelium and inflamed tissues, and dysregulated TNFSF15 signaling has been associated with inflammatory bowel disease, autoimmune disorders, and tumor-associated vascular changes. In oncology research, VEGI has been studied for its capacity to suppress tumor angiogenesis by limiting vascular support within the tumor microenvironment.

Because TL1A bridges vascular biology and immune activation, it remains an important target for studies examining endothelial-immune interactions and cytokine-driven inflammation in both normal and disease contexts.

Clone TLRM1-1 is a monoclonal antibody that recognizes TL1A and is suitable for detecting VEGI expression in immunohistochemical analyses of human tissue specimens.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TL1A antibody to be titered up or down for optimal performance.

Immunogen

A full length human recombinant protein was used as the immunogen for this TL1A antibody.

Storage

Store the TL1A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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