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Home >> Antibodies >> TJP2 Antibody / ZO-2

TJP2 Antibody / ZO-2 (R31077)

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  Catalog No Formulation Size Price (USD)  
Image R31077 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE mouse liver tissue with TJP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE mouse liver tissue with TJP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver tissue with TJP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human U-2 OS cells with TJP2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human RT4, 2) human A431, 3) human HepG2, 4) human MCF7, 5) human U-2 OS, 6) human HeLa, 7) human PC-3, 8) rat RH35 and 9) mouse HEPA1-6 cell lysate with TJP2 antibody. Expected molecular weight: 131-160 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt Q9Z0U1
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Limitations This TJP2 antibody is available for research use only.
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Description

Tight Junction Protein 2, also known as Zona Occludens 2 or ZO-2, is a protein that in humans is encoded by the TJP2 gene. Tight junction proteins (TJPs) belong to a family of membrane-associated guanylate kinase (MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the TJP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of mouse Tight Junction Protein 2 (AHSKRGYYSQPSRYRDTEL) was used as the immunogen for this TJP2 antibody (100% rat homology).

Storage

After reconstitution, the TJP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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