TIP49A Antibody | RuvB-like 1 | RQ7245 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	RQ7245	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

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IHC staining of FFPE human ovarian cancer tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human spleen tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human thyroid cancer tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung adenocarcinoma tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colorectal adenocarcinoma tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human A549 cells with TIP49A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) human K562, 2) human 293T, 3) human HT1080, 4) monkey COS-7, 5) human MCF7, 6) human Daudi, 7) human MOLT4, 8) human HEL, 9) rat testis, 10) rat C6, 11) mouse testis and 12) mouse NIH 3T3 cell lysate with TIP49A antibody. Predicted molecular weight ~50 kDa.

Flow cytometry testing of human HeLa cells with TIP49A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TIP49A antibody.

Immunofluorescent staining of FFPE human colon cancer tissue with TIP49A antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Immunoprecipitation of TIP49A protein from 500ug of human K562 whole cell lysate with 2ug of TIP49A antibody.

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat, Monkey
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	Q9Y265
Localization	Nuclear, cytoplasmic
Applications	Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml Flow Cytometry : 1-3ug/million cells Direct ELISA : 0.1-0.5ug/ml Immunofluorescence : 2ug per 500ug of lysate
Limitations	This TIP49A antibody is available for research use only.
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Description

TIP49A antibody targets RuvB-like 1 (RUVBL1), also known as TIP49A, a highly conserved AAA ATPase that functions as a multifunctional regulator of chromatin remodeling, transcription, and macromolecular complex assembly. RUVBL1 localizes predominantly to the nucleus, with additional cytoplasmic distribution depending on cellular context, reflecting its involvement in diverse nuclear processes. As a member of the AAA ATPase family, RUVBL1 forms hexameric and dodecameric complexes that provide ATP-dependent remodeling activity required for the stability and function of several essential protein assemblies.

Functionally, RUVBL1 is a core component of multiple chromatin-associated complexes, including INO80 and TIP60 histone acetyltransferase complexes, where it contributes to chromatin remodeling, DNA damage repair, and transcriptional regulation. Through these interactions, RUVBL1 influences gene expression programs linked to cell cycle progression, differentiation, and stress responses. RUVBL1 is broadly expressed across tissues, consistent with its fundamental role in maintaining nuclear organization and transcriptional control. A TIP49A antibody supports studies examining chromatin dynamics and transcriptional regulation.

RUVBL1 also plays an important role in the assembly and maturation of large macromolecular complexes, including small nucleolar ribonucleoproteins and RNA polymerase-associated factors. Its ATPase activity is required for proper complex formation and functional stability, linking energy-dependent remodeling to nuclear architecture and RNA processing pathways. Disruption of RUVBL1 function can impair DNA repair efficiency, transcriptional fidelity, and cellular viability, highlighting its essential nature. A TIP49A antibody enables investigation of RUVBL1 expression and localization in nuclear regulatory pathways.

From a biological and disease-relevance perspective, RUVBL1 has been extensively studied in cancer biology, where elevated expression is associated with increased proliferation and altered transcriptional programs. RUVBL1-dependent chromatin remodeling supports oncogenic signaling pathways and stress tolerance in rapidly dividing cells. Beyond cancer, RUVBL1 also contributes to developmental and homeostatic processes that rely on precise control of gene expression and chromatin structure.

At the molecular level, RUVBL1 is encoded by the RUVBL1 gene and produces a protein of approximately 456 amino acids. The protein contains conserved Walker A and Walker B motifs required for ATP binding and hydrolysis, which drive conformational changes necessary for remodeling activity. Regulation of RUVBL1 function depends on complex assembly, post-translational modification, and cellular context. A TIP49A antibody supports research applications focused on chromatin remodeling, transcriptional regulation, and nuclear complex biology, with NSJ Bioreagents providing reagents intended for research use.

Application Notes

Optimal dilution of the TIP49A antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids Q13-D23) was used as the immunogen for the TIP49A antibody.

Storage

After reconstitution, the TIP49A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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TIP49A Antibody / RuvB-like 1 / RUVBL1 (RQ7245)

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