TIP49A Antibody / RuvB-like 1 / RUVBL1 (RQ7245)

IHC staining of FFPE human ovarian cancer tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human spleen tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human thyroid cancer tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung adenocarcinoma tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colorectal adenocarcinoma tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human A549 cells with TIP49A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) human K562, 2) human 293T, 3) human HT1080, 4) monkey COS-7, 5) human MCF7, 6) human Daudi, 7) human MOLT4, 8) human HEL, 9) rat testis, 10) rat C6, 11) mouse testis and 12) mouse NIH 3T3 cell lysate with TIP49A antibody. Predicted molecular weight ~50 kDa.

Flow cytometry testing of human HeLa cells with TIP49A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TIP49A antibody.

Immunofluorescent staining of FFPE human colon cancer tissue with TIP49A antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.