Western blot testing of human 1) placenta, 2) K562, 3) T-47D, 4) Caco-2, 5) HeLa, 6) ThP-1, 7) A431 and 8) A549 lysate with TIMM17A antibody at 0.5ug/ml. Predicted molecular weight ~18 kDa.
Western blot testing of 1) rat heart, 2) rat lung, 3) rat liver, 4) rat brain, 5) mouse heart, 6) mouse lung, 7) mouse liver, 8) mouse brain and 9) mouse Neuro-2a lysate with TIMM17A antibody at 0.5ug/ml. Predicted molecular weight ~18 kDa.
IHC staining of FFPE human breast cancer with TIMM17A antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with TIMM17A antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with TIMM17A antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat lung with TIMM17A antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Flow cytometry testing of human A431 cells with TIMM17A antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TIMM17A antibody.
IHC staining of FFPE mouse lung tissue with TIMM17A antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IF/ICC staining of FFPE human A549 cells with TIMM17A antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IF/ICC staining of FFPE human HeLa cells with TIMM17A antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.