TIM-1 Antibody / KIM-1 / HAVCR1 (R30715)

Immunohistochemical staining using TIM-1 antibody on FFPE rat kidney tissue sections. Heat-induced epitope retrieval was performed using EDTA buffer (pH 8.0) prior to antibody incubation. TIM-1 antibody shows strong membranous and cytoplasmic staining in renal tubular epithelial cells, consistent with expression of T cell immunoglobulin and mucin domain-containing protein 1 in kidney tissue. Detection was performed using an HRP-conjugated secondary antibody with DAB as the chromogen, and nuclei were counterstained with hematoxylin.

Immunohistochemical staining using TIM-1 antibody on FFPE rat testis tissue sections. Sections were subjected to heat-induced epitope retrieval by steam treatment in pH 6 citrate buffer for 20 minutes prior to antibody incubation. TIM-1 antibody shows cytoplasmic and membranous staining in seminiferous tubule-associated cells, consistent with expression of T cell immunoglobulin and mucin domain-containing protein 1 in testicular tissue. Nuclei were counterstained with hematoxylin.

Western blot analysis using TIM-1 antibody. Lane 1: rat kidney tissue lysate. The predicted molecular weight of TIM-1 (HAVCR1 / KIM-1) is ~34 kDa, while TIM-1 antibody detects a prominent band at ~60 kDa on SDS-PAGE, consistent with the mature, glycosylated form of this type I transmembrane glycoprotein and previously reported migration of KIM-1 near this size range.