TIGIT Antibody / Broad Epitope Detection Antibody (RQ6213)

TIGIT Antibody Mouse Spleen IHC. Immunohistochemistry analysis of FFPE mouse spleen tissue using TIGIT Antibody demonstrates HRP-DAB brown membranous and cytoplasmic staining in subsets of lymphoid cells within the splenic parenchyma, consistent with TIGIT expression on activated T cells and NK cells, and reflecting broad epitope detection across immune cell populations, while surrounding cells show lower signal; nuclei are counterstained blue. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.

TIGIT Antibody Rat and Mouse Tissue WB. Western blot analysis of rat spleen, rat plasma, and mouse spleen lysates using TIGIT Antibody detects a band at the predicted molecular weight of approximately 26 kDa, consistent with TIGIT expression in lymphoid tissues, with slightly higher apparent molecular weight likely reflecting glycosylated forms of this immune checkpoint receptor, and supporting broad epitope detection across species and sample types.

TIGIT Antibody RAW264.7 FACS. Flow cytometry analysis of mouse RAW264.7 cells using TIGIT Antibody shows a clear rightward shift in fluorescence intensity compared to cells alone and isotype control, indicating TIGIT expression on the cell surface consistent with its role as an immune checkpoint receptor, and supporting broad epitope detection across immune cell populations.