TIE2 Antibody / TEK tyrosine kinase (RQ5554)

Immunofluorescence analysis of TIE2 / TEK tyrosine kinase antibody in human U-2 OS cells. FFPE human U-2 OS sections show cytoplasmic and membranous green fluorescence consistent with TIE2 expression, while nuclei are counterstained with DAPI (blue). Signal appears distributed throughout the cytoplasm with peripheral accentuation suggestive of membrane-associated localization. Heat induced epitope retrieval was performed in 10 mM citrate buffer, pH 6.0, by boiling for 20 minutes followed by cooling prior to antibody incubation.

Immunohistochemistry analysis of TIE2 / TEK tyrosine kinase antibody in human ovarian cancer tissue. FFPE human ovarian carcinoma sections demonstrate HRP-DAB brown staining primarily in vascular endothelial cells within the tumor stroma, with membranous and cytoplasmic signal highlighting microvascular structures. Tumor epithelial cells show minimal to weak staining compared to endothelial components. Nuclear hematoxylin counterstain is visible in blue. Heat induced epitope retrieval was performed in 10 mM citrate buffer, pH 6.0, by boiling tissue sections for 20 minutes followed by cooling prior to antibody incubation.

IHC staining of FFPE human tonsil with TIE2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Flow cytometry testing of human HeLa cells with TIE2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TIE2 antibody.

Western blot testing of human 1) HeLa, 2) U-87 MG, 3) U-2 OS, 4) A431, 5) PC-3 and 6) HL60 lysate with TIE2 antibody. Predicted molecular weight: ~126 kDa but may be observable at 130-165 kDa.

Western blot testing of mouse 1) lung and 2) Neuro-2a lysate with TIE2 antibody. Predicted molecular weight: ~126 kDa but may be observable at 130-165 kDa.

IHC staining of FFPE mouse testis with TIE2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE rat testis with TIE2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE rat lung with TIE2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE rat intestine with TIE2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.