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Home >> Antibodies >> TIA-1 Antibody

TIA-1 Antibody (R31316)

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  Catalog No Formulation Size Price (USD)  
Image R31316 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of TIA-1 antibody and Lane 1: Jurkat; 2: Raji; 3: CEM; 4: HT1080; 5: K562 cell lysate. Predicted molecular weight: ~43kDa and ~15kDa (granulocyte-associated isoform).
Western blot testing of TIA-1 antibody and Lane 1: Jurkat; 2: Raji; 3: CEM; 4: HT1080; 5: K562 cell lysate. Predicted molecular weight: ~43kDa and ~15kDa (granulocyte-associated isoform).
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P31483
Applications Western blot : 0.5-1ug/ml
Limitations This TIA-1 antibody is available for research use only.
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Description

TIA-1 (T-Cell Intracellular Antigen-1), also called WDM, is an RNA-binding protein involved in splicing regulation and translational repression. It is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte(CTL) target cells. By in situ hybridization, the gene is mapped to chromosome 2p13.3. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is though to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the TIA-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the N-terminus of human TIA1 (STQRSQDHFHVFV) was used as the immunogen for this TIA-1 antibody (100% mouse homology).

Storage

After reconstitution, the TIA-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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