DIO3 Antibody / Thyroid Hormone Metabolism Enzyme Antibody (F55036)

DIO3 Antibody Placenta WB. Western blot analysis of human placental tissue lysate using DIO3 Antibody / Thyroid Hormone Metabolism Enzyme Antibody demonstrates a prominent immunoreactive band at approximately 31–37 kDa, consistent with the expected molecular weight range of DIO3 (Iodothyronine Deiodinase 3). DIO3 is the principal thyroid hormone-inactivating enzyme responsible for converting thyroxine (T4) and triiodothyronine (T3) into inactive metabolites, thereby regulating tissue-specific thyroid hormone availability. The strong signal observed in placental tissue is consistent with the important role of DIO3 in controlling fetal thyroid hormone exposure and maintaining endocrine homeostasis during development. Predicted molecular weight: ~34 kDa. Observed molecular weight: 31–37 kDa.

DIO3 Antibody HepG2 WB. Western blot analysis of human HepG2 cell lysate using DIO3 Antibody / Thyroid Hormone Metabolism Enzyme Antibody demonstrates a distinct immunoreactive band at approximately 31–37 kDa, consistent with the expected molecular weight range of DIO3 (Iodothyronine Deiodinase 3). DIO3 is a selenoprotein enzyme that catalyzes the inactivation of thyroid hormones, thereby regulating local thyroid hormone availability and endocrine signaling. Expression of DIO3 in HepG2 cells is consistent with its role in tissue-specific control of thyroid hormone metabolism and cellular homeostasis. The observed band supports specific detection of endogenous DIO3 and demonstrates the utility of this antibody for western blot applications. Predicted molecular weight: ~34 kDa. Observed molecular weight: 31–37 kDa.

DIO3 Antibody Rat Skeletal Muscle WB. Western blot analysis of rat skeletal muscle tissue lysate using DIO3 Antibody / Thyroid Hormone Metabolism Enzyme Antibody demonstrates a distinct immunoreactive band at approximately 31–37 kDa, consistent with the expected molecular weight range of DIO3 (Iodothyronine Deiodinase 3). DIO3 functions as the primary thyroid hormone-inactivating deiodinase, catalyzing the conversion of thyroxine (T4) and triiodothyronine (T3) into inactive metabolites that regulate tissue-specific thyroid hormone signaling. Detection of DIO3 in skeletal muscle supports its role in controlling local endocrine activity, energy metabolism, and physiological adaptation within metabolically active tissues. The observed band demonstrates specific recognition of endogenous DIO3 and supports the utility of this antibody for western blot applications. Predicted molecular weight: ~34 kDa. Observed molecular weight: 31–37 kDa.

DIO3 Antibody Hepatocellular Carcinoma IHC. Immunohistochemical staining of FFPE human hepatocellular carcinoma tissue using DIO3 Antibody / Thyroid Hormone Metabolism Enzyme Antibody demonstrates cytoplasmic HRP-DAB brown staining within malignant hepatocyte-like tumor cells. The staining pattern is consistent with expression of DIO3 (Iodothyronine Deiodinase 3), the principal thyroid hormone-inactivating enzyme responsible for regulating local concentrations of thyroxine (T4) and triiodothyronine (T3). Positive immunoreactivity within neoplastic hepatic tissue highlights the role of DIO3 in controlling tissue-specific thyroid hormone signaling, metabolic regulation, and cellular growth pathways. These findings support the utility of DIO3 as a marker for studies of endocrine metabolism and thyroid hormone homeostasis in liver malignancies. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.