- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Thyroglobulin Antibody for IHC / TG IHC Antibody [clone MSVA-189R] (V6119)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V6119-100UG | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide | 100 ug | 599.50 | |
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V6119-20UG | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide | 20 ug | 324.50 |
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| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | MSVA-189R |
| UniProt | P01266 |
| Localization | Secreted |
| Applications | Immunohistochemistry (FFPE) : 1:100-1:200 |
| Limitations | This TG/Thyroglobulin antibody is available for research use only. |
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Thyroglobulin (TG) is a large secreted glycoprotein produced by thyroid follicular epithelial cells and stored within the colloid of thyroid follicles, where it functions as the precursor for thyroid hormone synthesis. The TG gene is located on chromosome 8q24 and encodes a glycosylated protein that undergoes extensive processing during thyroid hormone production. In histologic studies, TG expression is highly restricted to thyroid follicular epithelium, making thyroglobulin an important lineage marker of thyroid tissue. Thyroglobulin Antibody for IHC is therefore widely used in immunohistochemistry to detect TG expression and confirm thyroid follicular differentiation in tissue sections.
In immunohistochemistry, Thyroglobulin Antibody for IHC typically produces strong cytoplasmic staining in thyroid follicular epithelial cells that line thyroid follicles, reflecting the abundant synthesis and secretion of thyroglobulin within these cells. This cytoplasmic staining pattern is characteristic of differentiated thyroid follicular cells and is also observed in many thyroid-derived tumors that retain follicular differentiation. As a result, TG immunohistochemistry is frequently used in surgical pathology as a marker of thyroid origin. TG IHC antibody staining is particularly valuable when evaluating metastatic lesions in lymph nodes, lung, bone, or other tissues where the tissue of origin may not be immediately evident based on morphology alone. Demonstration of thyroglobulin expression by immunohistochemistry can therefore support the identification of metastatic thyroid carcinoma and help distinguish thyroid-derived tumors from non-thyroid malignancies.
Thyroglobulin Antibody for IHC clone MSVA-189R is a recombinant rabbit monoclonal antibody developed for sensitive detection of TG in formalin-fixed, paraffin-embedded tissue sections. In immunohistochemistry workflows, TG staining highlights the cytoplasm of thyroid follicular epithelial cells and tumor cells that maintain follicular differentiation. Tissue microarray analysis provides additional confirmation of the antibody's staining performance. Evaluation using human tissue microarray (TMA) sections containing a broad panel of normal and cancer tissues demonstrates strong, selective staining of thyroid follicular cells while showing minimal staining in non-thyroid tissues. Such human tissue microarray (TMA) validation across multiple tissue types supports the specificity of TG IHC antibody staining and reinforces its utility as a marker of thyroid follicular lineage in histologic studies.
Beyond diagnostic pathology, thyroglobulin immunohistochemistry is also widely used in research focused on thyroid gland biology, follicular epithelial cell differentiation, and mechanisms of thyroid tumor progression. Changes in TG expression may reflect the degree of tumor differentiation in thyroid cancers, with well differentiated tumors typically retaining thyroglobulin expression while poorly differentiated tumors may show reduced staining. Detection of TG expression using Thyroglobulin Antibody for IHC therefore provides an informative approach for studying thyroid lineage differentiation and tumor biology. By enabling visualization of thyroglobulin-positive cells in tissue sections and human tissue microarray (TMA) panels, TG IHC antibody reagents support detailed analysis of thyroid tissue architecture, tumor differentiation status, and thyroid-related disease processes.
1. Optimal dilution of the Thyroglobulin Antibody for IHC should be determined by the researcher.
2. This TG/Thyroglobulin antibody is recombinantly produced by expression in CHO cells.
3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Recombinant full-length human Thyroglobulin protein was used as the immunogen for the Thyroglobulin Antibody for IHC.
TG/Thyroglobulin antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.
TG antibody, Thyroglobulin protein antibody, Thyroid follicular cell marker antibody, Thyroid differentiation marker antibody
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