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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Thrombomodulin Antibody for IF / Endothelial Cell Immunofluorescence Marker (RQ7756)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ7756 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Rat |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | P07204 |
| Localization | Cell surface, cytoplasm, nucleus |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml Flow Cytometry : 1-3ug/million cells Immunoprecipitation : 2ug antibody/500ug lysate Direct ELISA : 0.1-0.5ug/ml |
| Limitations | This Thrombomodulin antibody is available for research use only. |
|
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Thrombomodulin (THBD) is a vascular endothelial membrane glycoprotein expressed on the luminal surface of endothelial cells that line blood vessels throughout the body. Thrombomodulin Antibody for IF is widely used in immunofluorescence microscopy to visualize endothelial cell membranes and analyze vascular structures using fluorescent antibody labeling. Because thrombomodulin is concentrated at the endothelial cell surface, immunofluorescence staining produces clear membrane-associated fluorescence signals that allow investigators to observe endothelial cells and vascular networks with high spatial resolution in fluorescence imaging experiments.
Thrombomodulin antibody, also referred to as THBD antibody and endothelial thrombomodulin antibody in the literature, detects a transmembrane receptor that regulates thrombin activity within the protein C anticoagulant pathway. The protein is localized along endothelial cell membranes where it participates in vascular anticoagulant signaling. This membrane localization makes thrombomodulin particularly well suited for immunofluorescence detection, where fluorescently labeled antibodies generate strong fluorescence signals outlining endothelial cell boundaries. In fluorescence microscopy experiments, this pattern allows investigators to clearly visualize endothelial cells that form vascular structures within tissues and cultured cell models.
In immunofluorescence imaging experiments, Thrombomodulin Antibody for IF typically produces membrane-associated fluorescence signals that highlight endothelial cells lining blood vessels. Under fluorescence microscopy or confocal microscopy, endothelial cells appear as continuous fluorescent outlines that define vascular structures within the tissue microenvironment. This fluorescence staining pattern allows researchers to examine endothelial cell morphology, endothelial junction organization, and vascular network architecture using high resolution fluorescence imaging techniques.
Immunofluorescence detection of thrombomodulin is frequently used in studies of vascular biology, endothelial cell differentiation, and angiogenesis. Fluorescence microscopy provides the ability to analyze endothelial cells in combination with additional fluorescent markers in multiplex imaging experiments. By combining thrombomodulin immunofluorescence staining with other endothelial or cellular markers, investigators can perform multi-color fluorescence imaging to study endothelial cell interactions, vascular organization, and cellular relationships within complex tissues.
This rabbit polyclonal antibody recognizes thrombomodulin expression and supports fluorescence-based detection of endothelial cells in research applications. When applied in fluorescence microscopy and confocal imaging experiments, Thrombomodulin Antibody for IF enables visualization of endothelial cell membranes and vascular structures through immunofluorescence staining, providing a valuable tool for analyzing endothelial cell organization and vascular architecture in fluorescence imaging studies.
Optimal dilution of the Thrombomodulin Antibody for IF / Endothelial Cell Immunofluorescence Marker should be determined by the researcher.
E. coli-derived recombinant human protein (amino acids Q29-L575) was used as the immunogen for the Thrombomodulin antibody for IF.
After reconstitution, the Thrombomodulin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Thrombomodulin antibody, THBD antibody, Endothelial thrombomodulin antibody, CD141 thrombomodulin antibody, Vascular endothelial thrombomodulin antibody
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