Thrombomodulin Antibody / Tumor Vascular Marker [clone THBD/1591] (V3407)

Thrombomodulin Antibody / Tumor Vascular Marker. Immunohistochemistry analysis of Thrombomodulin / THBD expression in human bladder carcinoma using clone THBD/1591. FFPE tumor tissue shows membranous HRP-DAB brown staining in tumor epithelial cells with additional staining of endothelial cells forming tumor-associated vascular structures, highlighting tumor vasculature within the tumor microenvironment. Antigen retrieval was performed by boiling sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 minutes followed by cooling at room temperature.

Thrombomodulin Antibody / Tumor Vascular Marker. Immunohistochemistry analysis of Thrombomodulin / THBD expression in human cervical carcinoma using clone THBD/1591. FFPE tumor tissue shows membranous HRP-DAB brown staining in tumor epithelial cells with additional labeling of endothelial cells lining tumor-associated blood vessels, highlighting tumor vasculature within the tumor microenvironment. Antigen retrieval was performed by boiling sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 minutes followed by cooling at room temperature.

Thrombomodulin Antibody / Tumor Vascular Marker. Immunohistochemistry analysis of Thrombomodulin / THBD expression in human placenta using clone THBD/1591. FFPE placental tissue shows membranous HRP-DAB brown staining in trophoblastic cells with additional staining of endothelial cells lining placental blood vessels, highlighting vascular structures relevant to tumor vascular marker studies of endothelial biology and angiogenesis. Antigen retrieval was performed by boiling sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 minutes followed by cooling at room temperature.

Western blot testing of 1) recombinant partial protein and 2) human ThP1 lysate with Thrombomodulin antibody (clone THBD/1591). Expected molecular weight ~60/100 kDa (unmodified/glycosylated).

SDS-PAGE Analysis of Purified, BSA-Free Thrombomodulin Antibody (clone THBD/1591). Confirmation of Integrity and Purity of the Antibody.

Thrombomodulin Antibody / Tumor Vascular Marker. Specificity validation of clone THBD/1591 using a HuProt(TM) human protein microarray containing more than 19,000 full-length human proteins. The antibody demonstrates highly selective binding to Thrombomodulin / THBD (CD141), a protein widely studied as a tumor vascular marker associated with endothelial cells forming tumor blood vessels. The dominant signal for THBD with minimal background reactivity supports the strong target specificity of the THBD/1591 monoclonal antibody.
Z- and S-score interpretation: The Z-score represents the strength of the fluorescent signal produced when the antibody binds to a specific protein on the HuProt(TM) array and is expressed as the number of standard deviations above the mean signal intensity of all proteins on the array. Proteins are ranked according to descending Z-scores. The S-score represents the difference between consecutive Z-scores and therefore reflects the relative specificity of the antibody for its intended target compared with other proteins on the array.