TGOLN2 Antibody / Trans-Golgi Network Marker Antibody (FY12425)

TGOLN2 Antibody Human Lung Cancer IF. Immunofluorescent staining of FFPE human lung cancer tissue using TGOLN2 Antibody. HIER was performed using pH 8 EDTA buffer, and sections were blocked with goat serum prior to staining. The tissue was incubated with rabbit anti-TGOLN2 antibody at 5 ug/ml overnight at 4°C. Cy3-conjugated goat anti-rabbit IgG (red) was used for detection, and nuclei were counterstained with DAPI (blue). This TGOLN2 Antibody demonstrates TGOLN2 expression within lung cancer tissue, supporting studies of trans-Golgi network function, vesicle trafficking, protein sorting, and intracellular transport pathways.

TGOLN2 Antibody Human Lung Cancer IHC. Immunohistochemical staining of FFPE human lung cancer tissue using TGOLN2 Antibody. HIER was performed using pH 8 EDTA buffer, and sections were blocked with goat serum prior to staining. The tissue was incubated with rabbit anti-TGOLN2 antibody at 2 ug/ml overnight at 4°C. An HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB substrate was used for detection. This TGOLN2 Antibody demonstrates TGOLN2 expression within lung cancer tissue, supporting studies of trans-Golgi network organization, vesicle trafficking, protein sorting, and intracellular transport pathways.

TGOLN2 Antibody Human Colon Cancer IHC. Immunohistochemical staining of FFPE human colon cancer tissue using TGOLN2 Antibody. HIER was performed using pH 8 EDTA buffer, and sections were blocked with goat serum prior to staining. The tissue was incubated with rabbit anti-TGOLN2 antibody at 2 ug/ml overnight at 4°C. An HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB substrate was used for detection. This TGOLN2 Antibody demonstrates TGOLN2 expression within colon cancer tissue, supporting studies of trans-Golgi network function, intracellular vesicle trafficking, protein sorting, and secretory pathway regulation.

TGOLN2 Antibody Human Colon Cancer IHC. Immunohistochemical staining of FFPE human colon cancer tissue using TGOLN2 Antibody. HIER was performed using pH 8 EDTA buffer, and sections were blocked with goat serum prior to staining. The tissue was incubated with rabbit anti-TGOLN2 antibody at 2 ug/ml overnight at 4°C. An HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB substrate was used for detection. This TGOLN2 Antibody demonstrates TGOLN2 expression in colon tumor cells and stromal-associated regions, supporting studies of trans-Golgi network organization, protein trafficking, vesicle transport, and regulation of secretory pathway function.

TGOLN2 Antibody Human Cancer Cell WB. Western blot analysis of human cancer cell lysates using TGOLN2 Antibody. Lane 1: human HepG2, Lane 2: human U251, Lane 3: human SiHa, Lane 4: human A549 cell lysate. The membrane was incubated with rabbit anti-TGOLN2 antibody at 0.5 ug/ml overnight at 4°C, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody and ECL detection. This TGOLN2 Antibody detects bands at approximately 90-100 kDa, consistent with the reported migration pattern of glycosylated TGN46/TGOLN2 protein, supporting studies of trans-Golgi network organization, protein trafficking, and vesicle transport pathways.

TGOLN2 Antibody Human Immune Cell FACS. Flow cytometric analysis of fixed and permeabilized human U937 cells using TGOLN2 Antibody at 1 ug/million cells. Cells were blocked with goat serum and compared against cells alone (red) and isotype control (green). A DyLight 488-conjugated goat anti-rabbit IgG secondary antibody was used for detection. TGOLN2 Antibody demonstrates a clear positive shift (blue), confirming intracellular TGOLN2 detection in U937 cells and supporting studies of trans-Golgi network function, protein sorting, vesicle trafficking, and secretory pathway regulation.