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Home >> Antibodies >> TGFa Antibody

TGFa Antibody [clone TGFA/1119] (V2888)

  Catalog No Formulation Size Price (USD)  
Image V2888-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2888-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2888SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V2888IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC testing of formalin-fixed, paraffin-embedded human testicular carcinoma stained with TGFa antibody (clone TGFA/1119).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name TGFA/1119
Purity Protein G affinity chromatography
UniProt P01135
Localization Cytoplasmic and extracellular (secreted)
Applications Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT
Limitations This TGFa antibody is available for research use only.
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Description

This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Application Notes

Optimal dilution of the TGFa antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human full-length TGF protein was used as the immunogen for the TGFa antibody.

Storage

Store the TGFa antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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