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- Email: info@nsjbio.com
In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Template commitment is established by the initial binding of TFIID to the TATA element of the promotor, a step which may be facilitated by TFIIA. TFIIB then acts as the bridge between TFIID and RNA polymerase II.
Optimal dilution of the TFIIB antibody should be determined by the researcher.
Recombinant full-length human protein was used as the immunogen for the TFIIB antibody.
Aliquot the TFIIB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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