TFE3 Antibody for Immunohistochemistry / Transcription Factor E3 IHC Antibody [clone TFE3/6849R] (V5328)

TFE3 Antibody for Immunohistochemistry. Immunohistochemistry analysis of FFPE human MiT translocation renal cell carcinoma tissue demonstrates strong nuclear HRP-DAB brown staining consistent with TFE3 (Transcription factor E3) localization in tumor cell nuclei. Nuclear staining is observed in tumor cells, reflecting the characteristic TFE3 IHC staining pattern associated with MiT family translocation renal cell carcinoma. Hematoxylin counterstain marks nuclei in blue. The recombinant rabbit monoclonal antibody clone TFE3/6849R was used at 2 ug/ml to detect nuclear TFE3 protein in tumor tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to staining.

TFE3 Antibody for Immunohistochemistry. Negative control immunohistochemistry analysis of FFPE human clear cell renal cell carcinoma tissue using the recombinant rabbit monoclonal TFE3 antibody clone TFE3/6849R shows absence of nuclear HRP-DAB brown staining in tumor cells, confirming lack of detectable TFE3 protein in this tissue type. Clear cell RCC typically lacks TFE3 overexpression associated with MiT family translocation tumors, and the absence of nuclear staining supports assay specificity. Hematoxylin counterstain marks nuclei in blue. The recombinant rabbit monoclonal antibody clone TFE3/6849R was used at 2 ug/ml. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to staining.