Tetherin Antibody / BST2 (RQ7154)

Tetherin Antibody / BST2 Antiviral Protein Antibody Human Liver Cancer IHC. Immunohistochemical staining of FFPE human liver cancer tissue using Tetherin Antibody / BST2 Antiviral Protein Antibody. Tetherin expression is detected predominantly within the cytoplasm and cell membrane of tumor cells, consistent with its role as an interferon-inducible antiviral restriction factor and cell surface glycoprotein. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

Tetherin Antibody / BST2 Antiviral Protein Antibody Hepatocellular Carcinoma IHC. Immunohistochemical staining of FFPE human liver cancer tissue using Tetherin Antibody / BST2 Antiviral Protein Antibody. Tetherin is detected predominantly within the cytoplasm and cell membrane of tumor cells, consistent with its role as an interferon-inducible antiviral restriction factor and cell surface glycoprotein. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

Tetherin Antibody / BST2 Antiviral Protein Antibody Cancer Cell Line WB. Western blot analysis of 1) human HeLa, 2) human SiHa, and 3) human A431 whole cell lysates using Tetherin Antibody / BST2 Antiviral Protein Antibody. Specific immunoreactive bands are detected at approximately 28-37 kDa, consistent with the heterogeneous glycosylation of BST2/Tetherin. Although the predicted molecular weight of the unglycosylated protein is approximately 20 kDa, glycosylated forms commonly migrate at higher apparent molecular weights in western blot analysis.

Western blot testing of human 1) HeLa and 2) HepG2 cell lysate with Tetherin antibody. Expected molecular weight: 20-40 kDa depending on glycosylation level.