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Home >> Antibodies >> Tenascin C Antibody

Tenascin C Antibody [clone T2H5] (V2602)

  Catalog No Formulation Size Price (USD)  
Image V2602-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2602-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2602SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V2602IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with Tenascin C antibody (T2H5) at 4ug/ml. Antigen retrieval in 10mM Tris with 1mM EDTA, pH 9.0; ABC detection system with DAB Chromogen. Note staining of connective tissue.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name T2H5
Purity Protein G affinity chromatography
UniProt P24821
Localization Connective tissue matrix
Applications Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT
Limitations This Tenascin C antibody is available for research use only.
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Description

In Western blotting, it reacts with two bands of ~MW of 210kDa and 300kDa, identified as two isoforms of Tenascin C. Specificity of this mAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linked hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.

Application Notes

Optimal dilution of the Tenascin C antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human breast carcinoma cells were used as the immunogen for the Tenascin C antibody.

Storage

Store the Tenascin C antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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