TEM8 Antibody / Tumor Endothelial Marker Antibody (RQ6934)

TEM8 Antibody Human Colon Cancer IHC. Immunohistochemical analysis of TEM8 Antibody / Tumor Endothelial Marker Antibody in FFPE human colon cancer tissue following heat-induced antigen retrieval in pH 8 EDTA buffer. The section was incubated with TEM8 antibody at 2 ug/ml and detected using an HRP-conjugated secondary antibody with DAB chromogen. Positive staining is observed in colon cancer cells, consistent with ANTXR1 expression. This TEM8 Antibody, a tumor endothelial marker antibody, is suitable for investigating receptor expression, tumor angiogenesis and cancer-associated endothelial biology.

TEM8 Antibody U2OS Cell IF. Immunofluorescence analysis of TEM8 Antibody / Tumor Endothelial Marker Antibody in U2OS cells following enzyme antigen retrieval. Cells were incubated with TEM8 antibody at 5 ug/ml and detected with a Fluoro488-conjugated secondary antibody (green), while nuclei were counterstained with DAPI (blue). The staining demonstrates punctate cytoplasmic and membrane-associated ANTXR1 expression. This TEM8 Antibody, a tumor endothelial marker antibody, is suitable for investigating receptor localization, tumor angiogenesis and extracellular matrix biology.

TEM8 Antibody Human, Rat and Mouse WB. Western blot analysis of TEM8 Antibody / Tumor Endothelial Marker Antibody detected a major band at approximately 80 kDa, consistent with the reported migration of glycosylated ANTXR1 despite a predicted molecular weight of 63 kDa. Lane 1: human HeLa; Lane 2: human A549; Lane 3: human SiHa; Lane 4: human SH-SY5Y; Lane 5: rat testis; Lane 6: rat lung; Lane 7: mouse testis; Lane 8: mouse lung lysate. The membrane was incubated with TEM8 antibody at 0.5 ug/ml followed by an HRP-conjugated secondary antibody. This TEM8 Antibody, a tumor endothelial marker antibody, detects endogenous ANTXR1 across human, rat and mouse samples.