TdT Antibody-Microarray Validated / DNA nucleotidylexotransferase [clone TDT/1393] (V8399)

TdT Antibody Thymus IHC. Immunohistochemistry of TdT antibody in human thymus tissue. Formalin-fixed, paraffin-embedded human thymus demonstrates strong nuclear staining in immature cortical thymocytes, consistent with Terminal deoxynucleotidyl transferase expression in developing T lymphoblasts. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to staining. The microarray-validated monoclonal antibody clone TDT/1393 was used as the primary antibody, showing distinct nuclear localization in thymic precursor cells.

TdT Antibody Human Thymus Tissue Immunohistochemistry. IHC staining of FFPE human thymus with TdT antibody (clone TDT/1393). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

TdT Antibody Microarray Specificity Validation. HuProt(TM) human protein microarray specificity analysis of TdT antibody. A HuProt(TM) microarray containing more than 19,000 full-length human proteins was probed with TdT antibody (clone TDT/1393) to assess binding specificity. The microarray-validated monoclonal antibody clone TDT/1393 demonstrates strong and selective signal for DNTT compared with other proteins on the array. Z-score represents the signal strength generated by antibody binding to a specific protein relative to the mean signal across the array, expressed in standard deviations above the mean. When proteins are ranked by descending Z-score, the S-score reflects the difference in standard deviations between consecutive targets and therefore indicates relative target specificity. The high Z- and S-scores observed for DNTT support the specificity profile of this TdT antibody.

TdT Antibody FFPE Thymus Tissue Immunohistochemistry. IHC analysis of FFPE human thymus with TdT antibody (clone TDT/1393). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

TdT Antibody Thymoma IHC. Immunohistochemistry of TdT antibody in human thymoma tissue. Formalin-fixed, paraffin-embedded human thymoma shows diffuse nuclear staining in neoplastic lymphoid cells, consistent with Terminal deoxynucleotidyl transferase expression in immature T cell populations. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to staining. The microarray-validated monoclonal antibody clone TDT/1393 was used as the primary antibody, demonstrating strong nuclear localization within tumor-associated thymocytes.