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Home >> Antibodies >> TDRKH Antibody

TDRKH Antibody [clone PCRP-TDRKH-1H2] (V9602)

  Catalog No Formulation Size Price (USD)  
Image V9602-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9602-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9602SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Immunofluorescent staining of PFA-fixed human HeLa cells using TDRKH antibody (green, clone PCRP-TDRKH-1H2) and phalloidin (red).
IHC staining of FFPE human ovarian carcinoma tissue with TDRKH antibody (clone PCRP-TDRKH-1H2) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
FACS staining of PFA-fixed human HeLa cells with TDRKH antibody (blue, clone PCRP-TDRKH-1H2), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free TDRKH antibody (clone PCRP-TDRKH-1H2) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using TDRKH antibody (clone PCRP-TDRKH-1H2). These results demonstrate the foremost specificity of the PCRP-TDRKH-1H2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name PCRP-TDRKH-1H2
Purity Protein A/G affinity
UniProt Q9Y2W6
Localization Cytoplasm
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This TDRKH antibody is available for research use only.
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Description

Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules.

Application Notes

Optimal dilution of the TDRKH antibody should be determined by the researcher.

Immunogen

Recombinant full-length human TDRKH protein was used as the immunogen for the TDRKH antibody.

Storage

Aliquot the TDRKH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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