- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
TDRKH Antibody / Microarray Specificity Validated Antibody [clone PCRP-TDRKH-1H2] (V9602)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V9602-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V9602-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V9602SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b |
| Clone Name | PCRP-TDRKH-1H2 |
| Purity | Protein A/G affinity |
| UniProt | Q9Y2W6 |
| Localization | Cytoplasm |
| Applications | Flow Cytometry : 1-2ug/million cells Immunofluorescence : 1-2ug/ml Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml |
| Limitations | This TDRKH Antibody / Microarray Specificity Validated Antibody is available for research use only. |
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Tudor and KH domain containing protein (TDRKH) is a cytoplasmic RNA-binding protein that plays a central role in piRNA pathway regulation and maintenance of genomic stability. TDRKH Antibody / Microarray Specificity Validated Antibody, clone PCRP-TDRKH-1H2, is a mouse monoclonal antibody developed for high-confidence detection of TDRKH, supported by protein microarray data demonstrating selective target recognition across a large panel of human proteins. TDRKH is predominantly localized in the cytoplasm with characteristic perinuclear enrichment, where it participates in RNA-processing complexes involved in small RNA biogenesis and post-transcriptional regulation. This antibody is part of a collection of Human Protein Microarray validated antibodies that have been screened for specificity across thousands of proteins.
TDRKH antibody, also referred to as Tudor and KH domain containing protein antibody or Tudor domain containing protein 2 antibody, recognizes a protein containing Tudor domains that mediate protein-protein interactions and KH domains that facilitate RNA binding. TDRKH functions in coordination with PIWI proteins and other components of the piRNA machinery, contributing to transposon silencing and preservation of genomic integrity, particularly in germline cells. Although highly expressed in reproductive tissues, TDRKH is also detectable in cultured cell systems, where it exhibits punctate cytoplasmic distribution consistent with RNA-associated granules and organelle-linked complexes.
This Microarray Specificity Validated Antibody is supported by protein microarray analysis demonstrating that the strongest binding signals correspond to TDRKH, with high Z-score values and clear separation from non-target proteins. The appearance of top-ranked signals for TDRKH reflects consistent recognition across replicate or isoform representations on the array and supports highly specific target binding. This level of validation is particularly important for RNA-binding proteins, which often share conserved domains that can increase the risk of off-target interactions, and therefore provides strong confidence in antibody selectivity in complex biological samples.
Microarray-based specificity validation provides a comprehensive and high-resolution assessment of antibody performance by evaluating binding across more than 19,000 full-length human proteins in a single experiment. This independent validation complements application-based data by confirming that observed staining patterns and detection signals are attributable to the intended target. In the case of TDRKH, this ensures that punctate cytoplasmic localization observed in immunofluorescence and tissue-based assays reflects true biological distribution rather than non-specific binding artifacts.
Complementary application data supports detection of TDRKH across multiple experimental formats. Immunofluorescence analysis demonstrates punctate cytoplasmic staining with prominent perinuclear enrichment in cultured cells, consistent with its association with RNA-processing complexes. Immunohistochemistry further confirms expression in tissue contexts, while flow cytometry enables detection at the cellular population level. Together, these data provide a multi-dimensional characterization of TDRKH expression while maintaining strong confidence in specificity supported by microarray validation.
TDRKH plays an important role in regulating RNA-protein interactions and maintaining genomic stability through control of small RNA pathways. Disruption of TDRKH function has been associated with defects in germ cell development and RNA processing, highlighting its biological importance. Given its role in RNA biology and the importance of accurate target detection, clone PCRP-TDRKH-1H2 represents a highly specific reagent for TDRKH analysis. A TDRKH antibody can be used to evaluate expression and localization in systems where specificity and confidence in target recognition are essential for reliable experimental outcomes.
This antibody is part of a broader antibody panel offered by NSJ Bioreagents.
Optimal dilution of the TDRKH Antibody / Microarray Specificity Validated Antibody should be determined by the researcher.
Recombinant full-length human TDRKH protein was used as the immunogen for the TDRKH antibody.
Aliquot the TDRKH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
TDRKH antibody, Tudor and KH domain containing protein antibody, Tudor domain containing protein 2 antibody, TDRKH microarray validated antibody, TDRKH protein antibody
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