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Home >> Antibodies >> TDG Antibody / Thymine-DNA glycosylase

TDG Antibody / Thymine-DNA glycosylase (RQ7493)

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  Catalog No Formulation Size Price (USD)  
Image RQ7493 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human HeLa cells with TDG antibody (green) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with TDG antibody (green) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human 293T, 2) human MCF7, 3) human K562, 4) human SH-SY5Y, 5) rat thymus, 6) rat brain, 7) mouse thymus and 8) mouse brain tissue lysate with TDG antibody. Predicted molecular weight ~46 kDa but may be observed at higher molecular weights due to ubiquitination and sumoylation.
Flow cytometry testing of fixed and permeabilized human MCF7 cells with TDG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TDG antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q13569
Localization Nuclear
Applications Western Blot : 0.5-1ug/ml
Flow Cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Immunofluorescence : 5ug/ml
Limitations This TDG antibody is available for research use only.
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Description

Thymine-DNA glycosylase (TDG) is a key enzyme in the base excision repair pathway, responsible for recognizing and excising mismatched thymine or uracil residues paired with guanine. By initiating DNA repair, TDG maintains genomic stability and prevents mutagenesis caused by spontaneous deamination or oxidative damage. Research using a TDG antibody helps explore its role in DNA repair and transcriptional regulation.

Beyond repair, TDG also interacts with transcription factors and chromatin-modifying complexes, influencing gene regulation and epigenetic processes. It has been linked to active DNA demethylation through the removal of oxidized methylcytosine intermediates, highlighting its importance in epigenetic control. Using a TDG antibody in studies aids in characterizing these regulatory mechanisms.

TDG has implications in cancer biology, as alterations in its activity may contribute to tumorigenesis by affecting genome integrity and gene expression patterns. A TDG antibody is valuable for assays such as western blot, immunohistochemistry, and chromatin studies. NSJ Bioreagents provides high-quality reagents to support TDG-related research.

Application Notes

Optimal dilution of the TDG antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids Q15-R370) was used as the immunogen for the TDG antibody.

Storage

After reconstitution, the TDG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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