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Home >> Antibodies >> TCP1 delta Antibody / CCT4

TCP1 delta Antibody / CCT4 (R32421)

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  Catalog No Formulation Size Price (USD)  
Image R32421 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) human HeLa, 2) human U-2 OS, 3) human SW620, 4) human MCF7, 5) rat brain, 6) rat liver, 7) mouse brain and 8) mouse liver tissue lysate with TCP1 delta antibody. Predicted molecular weight ~58 kDa.
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Western blot testing of 1) human HeLa, 2) human U-2 OS, 3) human SW620, 4) human MCF7, 5) rat brain, 6) rat liver, 7) mouse brain and 8) mouse liver tissue lysate with TCP1 delta antibody. Predicted molecular weight ~58 kDa.
IHC testing of FFPE human breast cancer tissue with TCP1 delta antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE mouse kidney with TCP1 delta antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE rat brain with TCP1 delta antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of frozen mouse brain tissue with TCP1 antibody.
IHC testing of frozen rat brain tissue with TCP1 antibody.
ICC testing of FFPE human SHG-44 cells with TCP1 delta antibody.
ICC testing of FFPE mouse Neuro-2a cells with TCP1 delta antibody.
IF/ICC staining of FFPE human U-2 OS cells with TCP1 delta antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-251 MG cells with TCP1 delta antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TCP1 delta antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt P50991
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Immunohistochemistry (Frozen) : 0.5-1ug/ml
Immunocytochemistry (FFPE) : 0.5-1ug/ml
Immunofluorescence (FFPE) : 2-4ug/ml
Flow Cytometry : 1-3ug/10^6 cells
Limitations This TCP1 delta antibody is available for research use only.
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Description

T-complex protein 1 subunit delta is a protein that in humans is encoded by the CCT4 gene. This gene is mapped to 2p15. The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha-transducin.

Application Notes

Optimal dilution of the TCP1 delta antibody should be determined by the researcher.

Immunogen

Amino acids R452-R539 of the human protein were used as the immunogen for the TCP1 delta antibody.

Storage

After reconstitution, the TCP1 delta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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