TCIRG1 Antibody / T-cell immune regulator 1 (FY12591)

Immunohistochemical staining of TCIRG1 using anti-TCIRG1 antibody. TCIRG1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-TCIRG1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate. Tonsil section shows scattered cytoplasmic/vesicular staining in macrophages and histiocytes within the germinal centers, consistent with TCIRG1 expression in antigen-presenting phagocytes.

Immunohistochemical staining of TCIRG1 using anti-TCIRG1 antibody. TCIRG1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-TCIRG1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate. Lung carcinoma section shows granular cytoplasmic and membranous staining in tumor-associated macrophages and scattered carcinoma cells, consistent with localization of the vacuolar-ATPase a3 subunit to acidified vesicular and plasma membrane regions.

Flow Cytometry analysis of THP-1 cells using anti-TCIRG1 antibody. Overlay histogram showing THP-1 cells stained with (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TCIRG1 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.