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Home >> Antibodies >> TCF3 Antibody / Transcription factor E2-alpha

TCF3 Antibody / Transcription factor E2-alpha (F54373)

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  Catalog No Formulation Size Price (USD)  
Image F54373-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54373-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human tonsil tissue with TCF3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human tonsil tissue with TCF3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) Jurkat and 2) Ramos cell lysate with TCF3 antibody. Expected molecular weight: 68-78 kDa.
Western blot testing of mouse cerebellum tissue lysate with TCF3 antibody. Expected molecular weight: 68-78 kDa.
Flow cytometry testing of human A549 cells with TCF3 antibody; Blue=isotype control, Green= TCF3 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P15923
Applications Western blot : 1:500-1:2000
Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Limitations This TCF3 antibody is available for research use only.
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Description

The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression.

Application Notes

The stated application concentrations are suggested starting points. Titration of the TCF3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 506-534 from the human protein was used as the immunogen for the TCF3 antibody.

Storage

Aliquot the TCF3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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