TBX5 Antibody | T-box protein 5 | FY13444 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	FY13444	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml	100 ug	439
Formulation

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Immunofluorescence analysis of TBX5 using anti-TBX5 antibody. TBX5 expression was examined in a paraffin-embedded section of human lung cancer tissue. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0), followed by blocking with goat serum. The tissue section was incubated with a rabbit anti-TBX5 antibody overnight at 4°C, then stained with a Cy3-conjugated goat anti-rabbit secondary antibody. Nuclei were counterstained with DAPI. Fluorescent signals were visualized by fluorescence microscopy, revealing prominent nuclear localization of TBX5 consistent with its role as a transcription factor.

Immunohistochemical staining of FFPE human lung cancer tissue with TBX5 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunohistochemical staining of FFPE human placental tissue with TBX5 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunohistochemical staining of FFPE mouse intestine tissue with TBX5 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunohistochemical staining of FFPE mouse heart tissue with TBX5 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunohistochemical staining of FFPE rat heart tissue with TBX5 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot analysis of TBX5 using anti-TBX5 antibody. Human whole cell lysates from HeLa, HepG2, MCF-7, and U2OS cells were resolved by 10% SDS-PAGE under reducing conditions and transferred to a nitrocellulose membrane. TBX5 was detected as a prominent band migrating at approximately 65-70 kDa across all tested cell lines. The predicted molecular weight of TBX5 based on amino acid sequence is approximately 58 kDa; the higher apparent molecular weight observed on SDS-PAGE is consistent with reported behavior of T-box transcription factors and may reflect post-translational modifications, altered SDS binding, or conformational properties that affect electrophoretic mobility rather than a difference in primary sequence.

Flow cytometry analysis of fixed and permeabilized human MCF7 cells with TBX5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TBX5 antibody.

Availability	1-2 days
Species Reactivity	Human, Mouse, Rat
Format	Lyophilized
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Immunogen affinity purified
Buffer	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
UniProt	Q99593
Localization	Nucleus, Cytoplasm
Applications	Immunohistochemistry : 2-5ug/ml Immunofluorescence : 5ug/ml Western Blot : 0.25-0.5ug/ml Flow Cytometry : 1-3ug/million cells ELISA : 0.1-0.5ug/ml
Limitations	This TBX5 antibody is available for research use only.
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Description

TBX5 antibody targets T-box protein 5, a transcription factor encoded by the TBX5 gene that plays a central role in embryonic development and organ specification. T-box protein 5 belongs to the T-box family of DNA-binding proteins, which are characterized by a conserved T-box domain that mediates sequence-specific binding to regulatory regions of target genes. TBX5 functions primarily in the nucleus, where it regulates gene expression programs essential for proper formation of the heart and upper limbs during early development.

Functionally, T-box protein 5 acts as a transcriptional regulator that coordinates developmental signaling pathways by activating or repressing downstream genes involved in cell fate determination, proliferation, and differentiation. TBX5 interacts with other transcription factors and cofactors to fine-tune gene expression in a spatially and temporally controlled manner. These interactions allow TBX5 to integrate multiple developmental cues and ensure coordinated morphogenesis of complex tissues.

TBX5 expression is tightly regulated and shows strong developmental stage and tissue specificity. It is prominently expressed in cardiac progenitor cells and developing limb structures, reflecting its essential role in cardiogenesis and limb patterning. In mature tissues, TBX5 expression is generally lower but can still be detected in specific cell populations where it contributes to maintenance of differentiated states and tissue-specific gene expression profiles. A TBX5 antibody is useful for examining these expression patterns in developmental and disease-related contexts.

From a clinical perspective, mutations or altered regulation of TBX5 are associated with congenital disorders, most notably Holt-Oram syndrome, which is characterized by cardiac defects and limb abnormalities. Dysregulation of TBX5-dependent transcriptional networks can disrupt normal developmental processes, leading to structural and functional defects. As a result, TBX5 has become an important target of interest in developmental biology, congenital disease research, and studies aimed at understanding transcription factor-driven gene regulation.

At the molecular level, T-box protein 5 contains conserved regions required for DNA binding and protein-protein interactions, enabling it to assemble transcriptional complexes at target promoters and enhancers. Post-translational modifications and interaction with cofactors can influence its transcriptional activity and regulatory specificity. TBX5 antibody reagents support research focused on developmental gene regulation, transcriptional control mechanisms, and disease-associated alterations in developmental pathways, with NSJ Bioreagents providing reagents intended for research use.

Application Notes

Optimal dilution of the TBX5 antibody should be determined by the researcher.

Immunogen

E.coli-derived human T-box protein 5 recombinant protein (amino acids K253-S518) was used as the immunogen for the TBX5 antibody.

Storage

After reconstitution, the TBX5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

TBX5 Antibody / T-box protein 5 (FY13444)

Description

Application Notes

Immunogen

Storage

Area of Research

Product Type

Protocols

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