TAS1R1 Antibody / Taste receptor type 1 member 1 (FY12039)

Western blot analysis of TAS1R1 using anti-TAS1R1 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: rat testis tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAS1R1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. The expected band size for TAS1R1 is at 93 kDa but the glycoprotein may be observed at higher molecular weights due to glycosylation.