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Home >> Antibodies >> TAGLN Antibody / Transgelin / SM22 alpha

TAGLN Antibody / Transgelin / SM22 alpha (F54870)

  Catalog No Formulation Size Price (USD)  
Image F54870-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54870-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human SK-BR-3 cell lysate with TAGLN antibody. Predicted molecular weight ~22 kDa.
Western blot testing of human uterus tissue lysate with TAGLN antibody. Predicted molecular weight ~22 kDa.
IHC testing of FFPE human skeletal muscle tissue with TAGLN antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human MDA-MB-468 cells with TAGLN antibody; Blue=isotype control, Green= TAGLN antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt Q01995
Localization Cytoplasmic
Applications Flow cytometry : 1:10-1:50 (1x10e6 cells)
Western blot : 1:500-1:1000
Immunohistochemistry (FFPE) : 1:50-1:100
Limitations This TAGLN antibody is available for research use only.
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Description

TAGLN is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.

Application Notes

The stated application concentrations are suggested starting points. Titration of the TAGLN antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 24-51 from the human protein was used as the immunogen for the TAGLN antibody.

Storage

Aliquot the TAGLN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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