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Home >> Antibodies >> TAG-72 Antibody

TAG-72 Antibody [clone TAG72/8317R] (V4072)

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  Catalog No Formulation Size Price (USD)  
Image V4072-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4072-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4072SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human colon carcinoma tissue with TAG-72 antibody (clone TAG72/8317R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human colon carcinoma tissue with TAG-72 antibody (clone TAG72/8317R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free TAG-72 antibody (clone TAG72/8317R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name TAG72/8317R
Purity Protein A/G affinity
Localization Cytoplasm, Cell surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This TAG-72 antibody is available for research use only.
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Description

Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This mAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma

Application Notes

Optimal dilution of the TAG-72 antibody should be determined by the researcher.

Immunogen

Purified TAG-72 protein was used as the immunogen for the TAG-72 antibody.

Storage

Aliquot the TAG-72 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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