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Home >> Antibodies >> SYP Antibody / Synaptophysin

SYP Antibody / Synaptophysin [clone MSVA-462R] (V6116)

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  Catalog No Formulation Size Price (USD)  
Image V6116-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 599.50
Image
V6116-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
Microvalidated Recrabbitmono
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Synaptophysin Rabbit Recombinant Monoclonal Antibody (MSVA-462R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-462R
UniProt P08247
Localization Cell junction, Cytoplasmic vesicle, Secretory vesicle, Synapse, Synaptic vesicle membrane, Synaptosome
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This SYP/Synaptophysin antibody is available for research use only.
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Application Notes

1. Optimal dilution of the SYP/Synaptophysin antibody should be determined by the researcher.

2. This SYP/Synaptophysin antibody is recombinantly produced by expression in human HEK293 cells.

Immunogen

A recombinant fragment (around amino acids 274-313) of human Synaptophysin (SYP) protein (exact sequence is proprietary) was used as the immuongen for the SYP/Synaptophysin antibody.

Storage

SYP/Synaptophysin antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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