Synaptophysin Antibody Rabbit Polyclonal / SYP Polyclonal Antibody (F54371)

Synaptophysin Antibody Rabbit Polyclonal. Immunohistochemistry analysis of FFPE human brain tissue demonstrates strong cytoplasmic HRP-DAB brown staining consistent with Synaptophysin (SYP) localization in presynaptic vesicle compartments of neuronal tissue. Positive staining highlights neuronal processes and synapse-rich neuropil regions where synaptic vesicles are densely distributed, while surrounding tissue structures show minimal background signal. Hematoxylin counterstain marks nuclei in blue. The rabbit polyclonal Synaptophysin antibody was used to detect synaptic vesicle protein expression in human brain tissue. Heat-induced epitope retrieval was performed by steaming sections in pH6 citrate buffer for 20 minutes followed by cooling prior to staining.

IHC testing of FFPE human pancreas tissue with Synaptophysin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Synaptophysin Antibody Rabbit Polyclonal for WB. Western blot analysis of human brain (lane 1) and human cerebellum (lane 2) lysates shows a distinct band at approximately 34-38 kDa, consistent with the predicted molecular weight of Synaptophysin (SYP). Synaptophysin is an integral synaptic vesicle membrane glycoprotein that is highly enriched in neuronal presynaptic terminals, and strong expression in brain and cerebellar tissue is expected due to the high density of synaptic vesicles in neuronal neuropil. The rabbit polyclonal Synaptophysin antibody detects SYP protein in human neuronal tissue.

Western blot testing of mouse 1) brain and 2) cerebellum lysate with Synaptophysin antibody. Predicted molecular weight: 34-38 kDa.

Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Synaptophysin antibody.