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Home >> Antibodies >> Synaptophysin Antibody / SYP

Synaptophysin Antibody / SYP [clone SYP/7976R] (V5336)

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  Catalog No Formulation Size Price (USD)  
Image V5336-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5336-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5336SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human adrenal tissue with Synaptophysin antibody (clone SYP/7976R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human adrenal tissue with Synaptophysin antibody (clone SYP/7976R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Synaptophysin antibody (clone SYP/7976R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name SYP/7976R
Purity Protein A/G affinity
UniProt P08247
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Synaptophysin antibody is available for research use only.
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Description

This recombinant rabbit monoclonal antibody recognizes a protein of 38kDa that is identified as synaptophysin. It is an N-glycosylated integral membrane protein found in neurons and endocrine cells. Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation.

Application Notes

Optimal dilution of the Synaptophysin antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 274-313) from the human protein was used as the immunogen for the Synaptophysin antibody.

Storage

Aliquot the Synaptophysin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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