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Home >> Antibodies >> Symmetric Dimethyl-Histone H4 Antibody / Symmetric / Arginine 3 / H4R3me2s

Symmetric Dimethyl-Histone H4 Antibody / Symmetric / Arginine 3 / H4R3me2s [clone RM459] (R20473)

  Catalog No Formulation Size Price (USD)  
Image R20473-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Microvalidated Recrabbitmono
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Western blot testing of acid extracts of human K562 cells (1) and recombinant Histone H4 (2) using recombinant Symmetric Dimethyl-Histone H4 antibody at 0.1 ug/ml.
Luminex analysis of Designer Recombinant Nucleosomes (dNucs) with the recombinant Symmetric Dimethyl-Histone H4 antibody. Biotinylated dNucs with unmodified or different modified arginine pre-bind to Luminex beads coated with streptavidin. The beads were then incubated with 1.6 ng/ml of recombinant Symmetric Dimethyl-Histone H4 antibody, follow by PE-conjugated goat anti-rabbit IgG secondary antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM459
Purity Protein A purified from animal origin-free supernatant
UniProt P62805
Applications Western blot : 0.1ug/ml-2ug/ml
Limitations This recombinant Symmetric Dimethyl-Histone H4 antibody is available for research use only.
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Description

This antibody reacts to Histone H4 symmetric dimethylated at Arginine 3 (R3me2s). No cross reactivity with unmodified Arginine 3, monomethylated or asymmetric dimethylated Arginine 3, or other methylated Arginine in Histone H4.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Symmetric Dimethyl-Histone H4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A symmetric dimethyl-Arginine 3 peptide from Histone H4 (Arg3) was used as the immunogen for the Symmetric Dimethyl-Histone H4 antibody.

Storage

Store the recombinant Symmetric Dimethyl-Histone H4 antibody at -20oC.

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