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Home >> Antibodies >> SYK Antibody / Spleen tyrosine kinase

SYK Antibody / Spleen tyrosine kinase (RQ5553)

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  Catalog No Formulation Size Price (USD)  
Image RQ5553 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) placenta and 2) ThP-1 lysate with SYK antibody. Predicted molecular weight ~72 kDa.
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Western blot testing of human 1) placenta and 2) ThP-1 lysate with SYK antibody. Predicted molecular weight ~72 kDa.
Western blot testing of mouse 1) thymus, 2) lung and 3) SP20 lysate with SYK antibody. Predicted molecular weight ~72 kDa.
Western blot testing of rat thymus lysate with SYK antibody. Predicted molecular weight ~72 kDa.
Flow cytometry testing of human ThP-1 cells with SYK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SYK antibody.
Flow cytometry testing of human A431 cells with SYK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SYK antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P43405
Applications Western blot : 0.25-0.5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This SYK antibody is available for research use only.
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Description

Spleen tyrosine kinase, also known as Syk, is an enzyme which in humans is encoded by the SYK gene. It is mapped to 9q22.2. This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Application Notes

Optimal dilution of the SYK antibody should be determined by the researcher.

Immunogen

A human recombinant protein (amino acids A10-N635) was used as the immunogen for the SYK antibody.

Storage

After reconstitution, the SYK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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