SYCP1 Antibody / Synaptonemal complex protein 1 (FY12055)

Western blot analysis of SYCP1 using anti-SYCP1 antibody. Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human THP-1 whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse TM4 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYCP1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. Expected size for full-length SYCP1 ~114 kDa. In our tumor-cell‐line lysates we detect a band at ~120-130 kDa and an additional band at ~150 kDa. The ~120-130 kDa band aligns with literature; the ~150 kDa band may represent a modified, aggregated or altered form of SYCP1 and has not been widely documented in published Western blots for SYCP1.