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Home >> Antibodies >> SUZ12 Antibody

SUZ12 Antibody (RQ5598)

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  Catalog No Formulation Size Price (USD)  
Image RQ5598 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human breast cancer with SUZ12 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast cancer with SUZ12 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human ThP-1 cells with SUZ12 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SUZ12 antibody.
Western blot testing of 1) human HL-60, 2) rat C6 and 3) mouse SP2/0 lysate with SUZ12 antibody. Expected molecular weight 83~95 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q15022
Applications Western blot : 0.25-0.5ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This SUZ12 antibody is available for research use only.
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Description

Polycomb protein SUZ12 is a protein that in humans is encoded by the SUZ12 gene. It is mapped to 17q11.2. This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.

Application Notes

Optimal dilution of the SUZ12 antibody should be determined by the researcher.

Immunogen

A human recombinant protein (amino acids K72-K483) was used as the immunogen for the SUZ12 antibody.

Storage

After reconstitution, the SUZ12 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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