SUN1 Antibody / Sad1/UNC-84 domain-containing protein 1 Antibody (FY12319)

SUN1 Antibody in Human Jurkat T Cells FACS. Flow cytometry analysis of fixed and permeabilized human Jurkat T cells with SUN1 Antibody / Sad1/UNC-84 Domain-Containing Protein 1 Antibody at 1 ug/million cells. Cells were fixed with 4% paraformaldehyde, permeabilized and blocked with 10% normal goat serum before incubation with the primary antibody for 30 min at 20oC. DyLight 488-conjugated goat anti-rabbit IgG was used as the secondary antibody at 5-10 ug/million cells for 30 min at 20oC. The blue histogram represents SUN1 staining, green represents the isotype control and red represents unstained cells. This SUN1 Antibody demonstrates intracellular detection of Sad1/UNC-84 domain-containing protein 1 in human Jurkat T cells.

SUN1 Antibody Human Cell WB. Western blot demonstrates detection of SUN1 in human A431, Jurkat, K562, and HeLa cell lysates. A prominent band is observed at approximately 100 kDa, slightly above the predicted molecular weight of approximately 87 kDa. This higher apparent molecular weight is consistent with published reports describing post-translationally modified and alternatively spliced SUN1 isoforms. This SUN1 Antibody is suitable for studies of SUN1, a nuclear envelope protein involved in LINC complex organization and nuclear-cytoskeletal coupling.