SUMO2/3 Antibody (F42027)

Fluorescent image of SH-SY5Y cells stained with SUMO2/3 antibody diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Fluorescent image of HeLa cells stained with SUMO2/3 antibody was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Fluorescent image of U251 cells stained with SUMO2/3 antibody. Ab was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels). Top: lysate tested with ERM Ab. Center: IP with FLAG Ab followed by WB with NSJ# F42008 SUMO antibody. Bottom: IP with FLAG Ab followed by WB with NSJ# F42027 SUMO2/3 antibody. (*) represents immunoprecipitated ERM-like forms detected by SUMO Abs.

Western blot analysis of lysate from (1) 293T, (2) HeLa, (3) HL-60, (4) Jurkat cell lines and (5) rat liver tissue using SUMO2/3 antibody at 1:1000.

SUMO2/3 antibody western blot analysis in (10 U251 cells, (2) mouse liver and (3) rat liver lysate.

Western blot testing of SUMO2/3 antibody and 293T cell lysate either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO3 gene.

IHC testing of human hepatocarcinoma stained with SUMO2/3 antibody.