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Home >> Antibodies >> SUMO2/3 Antibody

SUMO2/3 Antibody (F42018)

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  Catalog No Formulation Size Price (USD)  
Image F42018-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F42018-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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SUMO2/3 antibody western blot analysis in (1) 293 and (2) HeLa lysate
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SUMO2/3 antibody western blot analysis in (1) 293 and (2) HeLa lysate
Fluorescent confocal image of HeLa cell stained with SUMO2/3 antibody at 1:25. SUMO2/3 immunoreactivity is localized to the nucleus.
IHC analysis of FFPE human hepatocarcinoma tissue stained with SUMO2/3 antibody
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat, Bovine, Pig, Primate, Chicken, Zebrafish, Hamster, Xenopus
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P55854
Applications Western blot : 1:1000
Immunofluorescence : 1:10-1:50
IHC (Paraffin) : 1:10-1:50
Limitations This SUMO2/3 antibody is available for research use only.
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Description

SUMO2 and SUMO3 are members of the SUMO (small ubiquitin-like modifier) protein family. This protein family functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. In vertebrates, three members of the SUMO family have been described, SUMO 1 and the functionally distinct homologues SUMO 2 and SUMO 3. SUMO modification sites present in the N terminal regions of SUMO 2 and SUMO 3 are utilized by SAE1/SAE2 (SUMO E1) and Ubc9 (SUMO E2) to form polymeric chains of SUMO 2 and SUMO 3 on protein substrates, a property not shared by SUMO 1.

Application Notes

Titration of the SUMO2/3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 53-84 from human SUMO3 was used as the immunogen for this SUMO2/3 antibody.

Storage

Aliquot the SUMO2/3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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