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Home >> Antibodies >> STRADA Antibody / LYK5

STRADA Antibody / LYK5 (F54391)

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  Catalog No Formulation Size Price (USD)  
Image F54391-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54391-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Immunofluorescent staining of human A549 cells with STRADA antibody (green) and anti-Actin (red).
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Immunofluorescent staining of human A549 cells with STRADA antibody (green) and anti-Actin (red).
IHC testing of FFPE human brain tissue with STRADA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) human 293, 2) human MOLT-4 and 3) mouse brain lysate with STRADA antibody. Predicted molecular weight ~48 kDa.
Western blot testing of 1) human 293, 2) human HepG2, 3) human MOLT-4, 4) mouse NIH 3T3 and 5) mouse brain lysate with STRADA antibody. Predicted molecular weight ~48 kDa.
Flow cytometry testing of human SH-SY5Y cells with STRADA antibody; Blue=isotype control, Green= STRADA antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q7RTN6
Localization Nuclear, cytoplasmic
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:25
Immunofluorescence : 1:25
Flow cytometry : 1:25 (1x10e6 cells)
Limitations This STRADA antibody is available for research use only.
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  • Applications : WB, FACS, IF
    Reactivity : Human

Description

Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.

Application Notes

The stated application concentrations are suggested starting points. Titration of the STRADA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 303-346 from the human protein was used as the immunogen for the STRADA antibody.

Storage

Aliquot the STRADA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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